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Emerging molecular techniques in microbiology


Semesterangivelse: Forårs kursus Kurset udbydes i blok 4 Kurset udbydes i skemagruppe A Kurset giver 7,5 ETCS point

 


Udgave: Forår 2013 NAT
ECTS points: 7,5
Point: 7,5
Blokstruktur: 4. blok
Skemagruppe: A
Institutter: Biologisk Institut
Uddannelsesdel: Kandidat niveau
Kontaktpersoner: Lars Hestbjerg Hansen, Sektion for Mikrobiologi, tlf. 352 2059, email: hestbjerg@bio.ku.dk
Andre undervisere: Mette Burmølle, Søren Sørensen, Anders Priemé, Niels-Ulrik Frigaard, Qunxin She
Skema- oplysninger:  Vis skema for kurset
Samlet oversigt over tid og sted for alle kurser inden for Lektionsplan for Det Naturvidenskabelige Fakultet Forår 2013 NAT
Undervisnings- form: 6 hours lectures, 36 hours discussion classes / practical exercises.
Formål: To provide the students with a user-level knowledge of the latest molecular techniques, with special focus on the analysis of microorganisms and their activity in complex environments with high bacterial diversity. The course will provide an in-depth understanding of important aspects of the genetic organization, adaptability and diversity of bacteria and archaea.
Indhold: The course consists of 6 lectures, 6 practical exercises and 12 discussion classes focusing on important topics in current microbial research. Topics covered include: diversity of microorganisms in complex environments e.g the human gut and multispecies biofilm, relationship between function and diversity, soil microbial activity and heavy metal resistance, the prevalence of opportunistic pathogenic bacteria in natural environments. The course will provide the students with both theoretical and hands-on experience with some of our new in-house technologies like Next Generation Sequencing, DNA microarray, Flow cytometry, Fluorescence In-Situ Hybridisation (FISH) and Real-time Quantitative PCR. These techniques will be used to describe the impact of anthropogenic contamination on a selected microbial community. As an integrated part of the course, the students will conduct a practical research project and use the data for writing a scientific paper.
Målbeskrivelse: By the end of the course students are expected to be able:
  • To describe the diversity microbial communities in natural environments e.g the human gut and biofilm.
  • To explain the interactions within bacterial communities.
  • To use the course curriculum to hypothesize problems in microbial ecology and design experiments to examine the hypothesis using molecular techniques.
  • To evaluate methods for identifying and accessing the microbial communities.
  • To explain in detail and evaluate different DNA based techniques and detection methods.
  • To use, compare and criticize the different bacterial fingerprinting techniques described in the course.
  • To explain in detail and evaluate techniques such as DNA sequencing, RT-qPCR, DNA microarray, metagenomics, and FISH; and to evaluate which of the molecular techniques are most suited to use in different situations.
  • To discuss, present, put into perspective and criticize original microbial research papers.
  • To write an original scientific paper based on data from the molecular techniques used and described in the course
  • Lærebøger: Various reprints of scientific papers and reviews. Literature can be downloaded from the course homepage
    Tilmelding: Enrolment at KUnet from 15 November to 1 December.
    Faglige forudsætninger: An introductory course in microbiology is recommended.
    Eksamensform: Each student will have to present and discuss a self-written scientific paper based on the data acquired on the course. The paper and a final 30-minute oral examination with external censorship will be rated by the 7-scale.
    Re-examination: New presentation of improved scientific paper and new oral exam.
    Eksamen: Aflevering af opgave d. 13. juni 2013 og mundtlig prøve d. 20. juni 2013.
    Reeksamen: Aflevering af opgave d. 19. august 2013 og mundtlig prøve d. 22. august 2013.
    Kursus hjemmeside:
    Bemærkninger: Max. 24 participants.
    The course is part of the qualification profile Microbiology. For all courses of the qualification profiles, see Course Portal for Department of Biology.
    The course is part of the Copenhagen School of Microbiology - COSMIC.
    Undervisnings- sprog: Engelsk
    Sidst redigeret: 11/12-2012



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