|
| Udgave: |
Efterår 2012 NAT |
| Point: |
15,0 |
| Blokstruktur: |
1. blok |
| Fagområde: |
bio |
| Institutter: |
Department of Biology |
| Uddannelsesdel: |
Bachelor niveau |
| Kontaktpersoner: |
Per Amstrup Pedersen, Department of Biology, August Krogh Building, Universitetsparken 13, phone +45 3532 1667, e-mail: papedersen@bio.ku.dk |
| Andre undervisere: |
Rasmus Hartmann-Petersen og Henriette Pilegaard |
| Skema- oplysninger: |
 Vis skema for kurset
Samlet oversigt over tid og sted for alle kurser inden for Lektionsplan for Det Naturvidenskabelige Fakultet Efterår 2012 NAT |
| Undervisnings- periode: |
Weeks 36-45 |
| Undervisnings- form: |
Lectures: 28 hours
Problem solving: 21 hours
Laboratory experiments: 60 hours. |
| Formål: |
Professional: The student gains core competences within theoretical and practical modern gene technology. The degree of confidence with modern gene technology/molecular biology obtained during the course allows him/her to design gene technology/molecular biology experiments based upon known gene technological methods and internet based tools. The student obtains experience with critical interpretation and treatment of original molecular biology data, and gets acquainted with work routines in a gene technology laboratory in relation to Danish and EU law on gene technology and genetically modified organisms (GMOs).
Practical: The student gets aquainted with practical laboratory routines fulfilling the requirements stated by the Danish and EU law on gene technology.
Personal: The student learns to collaborate in groups and prepare a written presentation and discussion of their obtained experimental data.
|
| Indhold: |
The theoretical part includes:
- A thorough review of gene technology methods
- Cellular control of macromolecular biosynthesis with emphasis on the experimental basis
- Generation of genome- and cDNA libraries
- Screening of libraries including expression cloning in Xenopus oocytes and micro organisms
- Genome-, trancriptome- and proteome analysis
- Production of transgenic animals
- Heterologous expression in bacteria, yeast/fungi, insect cells, mammalian cells and whole animals
The practical part includes:
- The most important methods to generate recombinant plasmids using restriction enzymes, Gateway® technology or homologous recombination
- Site-directed mutagenesis
- Real-time PCR for mRNA quantification and SNP analysis
- Application of reporter genes to study gene regulation and subcellular protein localisation
- Yeast 2-hybrid analysis
- Affinity purification of fusion proteins
- Pull-down experiments
- Fluorescence microscopy
- Internet based gene technology tools
- Danish and EU law on gene technology
|
| Målbeskrivelse: |
To obtain the mark 12 the student should
Be able to give a detailed description of and evaluate the gene technology tools presented during the course
Be able to apply the presented tools to design experiments with the potential to answer defined molecular biology questions
Be able to describe and explain individual steps in a presented gene technology manual
Be able to interpret data from the scientific literature and relate those to the content of the course
|
| Lærebøger: |
Robert F. Weaver, Molecular Biology (2011), Mc. Graw Hill, 5th edition, notes, theoretical problems and the laboratory manual. |
| Tilmelding: |
Registration for the course 15 May - 1 June at KUnet
|
| Faglige forudsætninger: |
The course is offered to students of Biology, Biochemistry, Molecular Biomedicine, Nanotechnology, Sports Science and related educations. |
| Formelle krav: |
Knowledge corresponding to the content of the courses: Basic Biochemistry, Basic Molecular Biology and Basic Cell Biology. |
| Eksamensform: |
A 4 hour written open book examination. Grading according to the Danish 7-grade scale with internal censorship.
Reexam: Same as ordinary exam. |
| Eksamen: |
Skriftlig prøve den 7. november 2012.
Reeksamen: Skriftlig prøve den 30. januar 2013. |
| Kursus hjemmeside: |
 |
| Undervisnings- sprog: |
Engelsk
|
| Sidst redigeret: |
22/5-2012 |