|
| Udgave: |
Efterår 2012 NAT |
| Point: |
7,5 |
| Blokstruktur: |
1. blok |
| Skemagruppe: |
B |
| Fagområde: |
andet |
| Institutter: |
Biologisk Institute |
| Uddannelsesdel: |
Bachelor niveau |
| Kontaktpersoner: |
Birthe B. Kragelund bbk@bio.ku.dk , 3532 2081 |
| Skema- oplysninger: |
3-hours teaching Tuesdays 13:15-16:00 and Fridays 9:15–12:00 in Copenhagen Biocenter,
Introduction and course kick-off: Monday 3 September, 12:15-13:00.
Essay writing: Week 40.
Essay assignment: Friday 30 September 13:15 -14:00; Essay presentations and oral presentations: Week 43.
|
| Skema- oplysninger: |
 Vis skema for kurset
Samlet oversigt over tid og sted for alle kurser inden for Lektionsplan for Det Naturvidenskabelige Fakultet Efterår 2012 NAT |
| Undervisnings- form: |
Subject-oriented teaching. A mixture of lecturing, problem solving, student seminars, computer assignments, and scientific discussions |
| Formål: |
The course is open for Biochemistry, Biology and Molecular Biomedicine students and includes chemistry, structure, function and biology of proteins. Students will obtain a theoretical understanding of protein biology, protein function and protein regulation. It is a requirement that the student has passed a basic course in protein science such as Biochemistry 2 (Biochemistry), Protein Chemistry and Enzymology (Molecular Biomedicine) or Almen biokemi 2 (Biology students). Students in pharmaceutical sciences are also welcome. |
| Indhold: |
Four weeks of theoretical teaching period with 2 x 3 hours teaching a week. Each 3-hours teaching session will be a mixture of lecturing, problem solving, student seminars, computer assignments, and scientific discussions. One week is reserved for obligatory oral presentations.
This course is theoretical and covers the biology, chemistry, structure and function of proteins in their biological environments. Subjects include: protein chemistry methods and strategies, fluorescent proteins as tools, redox potentials, intrisic disorder in protein science, proteome analysis, and enzyme mechanisms, protein metabolism in living cells, including in vivo folding, processing, enzyme systems and regulation, systems biology and proteomics. A basic knowledge of protein chemistry is required for admission to the (F) line. |
| Målbeskrivelse: |
In order to obtain the grade 12 the student should convincingly and accurately
Physical and chemical properties of proteins and amino acids
Demonstrate a general understanding of a selection of biophysical, spectroscopic, chemical and analytical methods
Demonstrate the ability to assess when to use which methods for solving a specific problem
Methods in analyses of structure and dynamics of proteins
Describe and understand basic methods in protein science such as SDS-PAGE, NATIVE-PAGE, ion-exchange chromatography, and gelfiltration
Describe and understand advanced methods in protein science in relation to interactions covering application of ITC, SPR and NMR spectroscopy in interaction analyses
Describe the use of fluorescence spectroscopy for analysis of protein
Describe the basic concepts of fluorescence resonance energy transfer
Understand the relative advantages of the above methods for protein structure and functional analysis
Oxidative stress and redox metabolism
Understand and describe basic concepts of thiol-disulphide reactions in disulphide exchange reactions and disulphide bond formation
Understand and describe the function and mechanism of selected antioxidant redox enzymes and systems
Understand and describe basic concepts of oxidation damage and reactive oxygen species
Calculate redox and reaction rate of thiols and disulfide equilibria
Regulated proteolysis in the immune system
Describe mechanisms of protein degradation in relation to proteasome structure and function
Describe and understand the proteolysis in the light of antigen presentation and immunological aspects
Apply the understanding of immunological techniques to practical problems in cellular protein chemistry
Intrisic disorder in protein science
Demonstrate insight into intrisic disorder of proteins
Describe and understand disorder characteristics
Describe the concepts of intrisic disorder in relation to membrane proteins and evaluate and analyses outputs
Demonstrate a thorough understanding of the structure/function relationship of disordered proteins
Integrate experimental and theoretical data in membrane protein structure analysis and integrate these in relation to pharmaceutical science
Knowledge on post translational modifications in relation to protein disorder
Evaluate protein stability and flexibility in relation to function
Describe thermodynamically the underlying physical chemistry in protein interactions and discuss these in terms of conformer selection mechanism of protein-protein interactions
From gene to function – bioinformatics and proteomics
Describe and understand the following terms: protein sequence convergence and divergence, orthologous and paralogous proteins, domain swapping, homology, sequence alignments, structural alignments, phylogenetic reconstruction, distance matrix, phylogenetic tree
Describe and understand concepts, strategies and methods in proteomics and functional genomics
Critically evaluate experimental results from proteomic analysis
Cite and understand the use of methods applied in proteomics and functional genomics including mass spectrometry, MS/MS, 2-D gel electrophoresis, protein and DNA micro array, fluorescence resonance energy transfer, yeast two-hybrid assay
Cite and understand the use of applied protein bioinformatics (BLAST homology searches)
Fluorescent proteins as tools
Describe and understand structure, function and application of fluorescent proteins
Design experiments based on this knowledge
Defining, attacking and presenting a scientific problem in protein chemistry (oral presentation)
Select and define a simple scientific research proposal in protein chemistry based on a selected set (1-3) of scientific subjects
Evaluate methods and theoretical approaches to address specific questions in relation to this research topic
Communicate verbally in a scientific language and present results in power points in a clear and informative way
Others
Analyze, evaluate and condense experimental data in protein science from combinations of all possible areas of curriculum to solve relevant protein science problems
Write a grant proposal in relation to a pre-defined subject provided by the lecturers of the course
Demonstrate written- and oral communication in a protein scientific language
Participate in a seminar on latest topics in protein science
To be able to attend the written exams in the Protein Science F course the students should have performed the following at a satisfactory level:
Written a grant proposal relating to a subject presented at the course after guidelines provided at the course
Presented the proposal orally in a 10 minutes presentation and discussed the content satisfactorily with the assigned lecturer. |
| Lærebøger: |
The course material will be a collection of original papers, extracts from textbooks and notes and laboratory manuals and can be downloaded from the course website. |
| Tilmelding: |
Via KUnet during the period 15 May - 1 June. |
| Faglige forudsætninger: |
Open to students of Biochemistry, Biology, Nanascoence, and Molecular Biomedicine who have passed all first year courses and half of the second year courses (corresponding to a total of 90 ECTS-points) of their curriculum. |
| Formelle krav: |
It is a requirement that a basic course in protein chemistry or protein biology has been passed, such as Biokemi2 (Biochemistry), Proteinkemi og Enzymology I (Molecular biomedicine), Almen biokemi 2 (Biology), Nanobio 2 (Nanoscience). |
| Eksamensform: |
30 minutes of oral exam without time for preparation. The oral exam will be initiated from the essay. Graded according to the danish 7 step scale, and with internal censorship.
Reexam: Same as the ordinary exam. |
| Eksamen: |
Kurset er aflyst. |
| Kursus hjemmeside: |
 |
| Kursus hjemmeside: |
Absalon via KUnet |
| Bemærkninger: |
The number of participants accepted is limited to 30. The course will not be held if the number of students is less than 15.
Teaching language will be English if non-Danish speaking students are present.
Credit for this course will not be given to students that have passed the former courses "Theoretical Protein Chemistry" or "Proteiner. Struktur og funktion".
|
| Undervisnings- sprog: |
Engelsk
|
| Sidst redigeret: |
26/6-2012 |